CRYO2022 Schedule


Monday, July 18 TIMESESSION 12:00 PM-5:00 PM Registration

Tuesday, July 19 TIMESESSION 8:30 AM-10:30 AM PLENARY SESSION 1Location: The Ballroom 4 Subsessions 10:30 AM-11:00 AM COFFEE BREAKLocation: O'Connell Suite 11:00 AM-1:00 PM PLENARY SESSION 2: IN MEMORIAM DAVID PEGGLocation: The Ballroom Sponsored By:Sylvatica Biotech 6 Subsessions 1:00 PM-2:00 PM LUNCHLocation: O'Connell Suite 2:00 PM-3:30 PM PLENARY SESSION 3: CELEBRATING 50 YEARS OF THE INSTITUTE FOR PROBLEMS OF CRYOBIOLOGY AND CRYOMEDICINE (IPCC) - HISTORY AND FUTURE OUTLOOKLocation: The Ballroom 4 Subsessions 3:30 PM-4:00 PM COFFEE BREAKLocation: O'Connell Suite 4:00 PM-6:20 PM PLENARY SESSION 3 CONT.: CELEBRATING 50 YEARS OF THE IPCC - OVERVIEW OF DISCIPLINES AND CURRENT RESEARCHLocation: The Ballroom 7 Subsessions 6:30 PM-8:30 PM CELEBRATING 50 YEARS OF THE IPCC - RECEPTIONLocation: O'Connell Suite

Wednesday, July 20 TIMESESSION 8:30 AM-10:30 AM SESSION 1: CRYOPROTECTANTS & ICE FORMATION 1Location: The Ballroom 5 Subsessions 8:30 AM-10:30 AM SYMPOSIUM 1: FERTILITY PRESERVATIONLocation: Trinity 1 4 Subsessions 8:30 AM-10:30 AM SYMPOSIUM 2: CRYOBIOTECHNOLOGY OF EXCEPTIONAL PLANT SPECIES: CHALLENGES AND SUCCESSESLocation: Trinity 2 4 Subsessions 10:30 AM-11:00 AM COFFEE BREAKLocation: O'Connell Suite 11:00 AM-1:00 PM SESSION 2: PRACTICAL CONCERNS AND CONSIDERATIONS IN BIOBANKINGLocation: The Ballroom 6 Subsessions 11:00 AM-1:00 PM SESSION 3: RECENT ADVANCES IN THE CRYOPRESERVATION OF OOCYTES AND OVARIAN TISSUELocation: Trinity 1 6 Subsessions 11:00 AM-12:40 PM SESSION 4: RECENT ADVANCES IN PLANT CRYOBIOTECHNOLOGY FOR PRESERVATION OF AGRICULTURAL AND HORTICULTURAL SPECIESLocation: Trinity 2 5 Subsessions 12:40 PM-1:00 PM SESSION 5: LYOPHILIZATION FOR PRESERVATIONLocation: Trinity 2 1 Subsession 1:00 PM-1:50 PM LUNCHLocation: O'Connell Suite 1:50 PM-3:30 PM SESSION 6: CRYOPROTECTANTS & ICE FORMATION 2Location: The Ballroom 5 Subsessions 1:50 PM-3:30 PM SYMPOSIUM 3: ORGAN PRESERVATION FOR TRANSPLANTATIONLocation: Trinity 1 4 Subsessions 3:30 PM-4:00 PM COFFEE BREAKLocation: O'Connell Suite 4:00 PM-6:20 PM SESSION 7: RECENT ADVANCES IN CELLULAR THERAPYLocation: The Ballroom 7 Subsessions 4:00 PM-6:00 PM SYMPOSIUM 3: ORGAN PRESERVATION FOR TRANSPLANTATION (CONTINUED)Location: Trinity 1 6 Subsessions 4:00 PM-6:20 PM SESSION 8: MODELING TO OPTIMIZE CRYOPRESERVATIONLocation: Trinity 2 7 Subsessions 6:30 PM-8:30 PM IN PERSON POSTER SESSIONLocation: O'Connell Suite

Thursday, July 21 TIMESESSION 8:00 AM-10:20 AM SESSION 10: COMPARATIVE SPERM CRYOPRESERVATIONLocation: Trinity 1 7 Subsessions 8:30 AM-10:30 AM SESSION 9: RECENT ADVANCES IN VITRIFICATIONLocation: The Ballroom 7 Subsessions 10:20 AM-11:00 AM COFFEE BREAKLocation: O'Connell Suite 11:00 AM-1:05 PM SYMPOSIUM 4: NOVEL TECHNOLOGIES AND THE FUTURE OF CRYOPRESERVATIONLocation: The Ballroom 5 Subsessions 11:00 AM-1:00 PM SESSION 11: CRYOPRESERVATION APPLIED TO AQUATIC ORGANISMSLocation: Trinity 1 6 Subsessions 1:00 PM-1:50 PM LUNCHLocation: O'Connell Suite 1:50 PM-3:30 PM COMPETITION: CRYSTAL AWARDLocation: The Ballroom 5 Subsessions 2:10 PM-2:30 PM SESSION 12: LEGAL AND ETHICAL CONSIDERATIONS IN CRYOBIOLOGYLocation: Trinity 1 2 Subsessions 2:30 PM-3:30 PM SESSION 13: COMPARATIVE EMBRYO CRYOPRESERVATIONLocation: Trinity 1 3 Subsessions 3:30 PM-4:00 PM COFFEE BREAKLocation: O'Connell Suite 4:00 PM-6:00 PM SESSION 14: TOOLS AND TECHNOLOGIES FOR CRYOPRESERVATION AND CRYOPRESERVATION 1Location: The Ballroom 6 Subsessions 4:00 PM-6:00 PM SESSION 15: LESSONS FROM NATURE: PLANT AND ANIMAL ADAPTATIONS TO COLDLocation: Trinity 1 6 Subsessions 6:15 PM-7:15 PM SOCIETY FOR CRYOBIOLOGY ANNUAL GENERAL MEETINGLocation: The Ballroom

Friday, July 22 TIMESESSION 8:10 AM-10:30 AM SESSION 16: NOVEL TOOLS AND TECHNOLOGIES FOR CRYOPRESERVATION AND CRYOPRESERVATION 2Location: The Ballroom 7 Subsessions 8:30 AM-10:30 AM SYMPOSIUM 5: ADVANCES IN THE CRYOPRESERVATION OF CONVENTIONAL BLOOD PRODUCTSLocation: Trinity 1 5 Subsessions 10:30 AM-11:00 AM COFFEE BREAKLocation: O'Connell Suite 11:00 AM-1:05 PM SYMPOSIUM 6: RECENT ADVANCES IN CELL THERAPEUTICSLocation: The Ballroom 5 Subsessions 11:00 AM-1:00 PM SESSION 17: RECENT ADVANCES IN CELL & TISSUE PRESERVATIONLocation: Trinity 1 6 Subsessions 1:00 PM-2:00 PM LUNCHLocation: O'Connell Suite 2:00 PM-3:15 PM AWARD SESSIONLocation: The Ballroom 2 Subsessions 2:00 PM-3:20 PM SESSION 18: RECENT ADVANCES IN PLANT CRYOBIOTECHNOLOGY FOR PRESERVATION OF WILD SPECIESLocation: Trinity 1 4 Subsessions 3:20 PM-4:00 PM COFFEE BREAKLocation: O'Connell Suite 4:00 PM-5:40 PM SYMPOSIUM 7: WHOLE ORGANISM CRYOPRESERVATIONLocation: The Ballroom 6 Subsessions 4:00 PM-5:00 PM SESSION 19: RECENT ADVANCES IN CRYOSURGERYLocation: Trinity 1 3 Subsessions 6:00 PM-6:30 PM CLOSING REMARKSLocation: The Ballroom 7:00 PM-10:00 PM CRYO2022 BANQUET




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PLENARY SESSION 1 8:30 AM -10:30 AMat The Ballroom Co-Chairs: Greg Fahy and Christiani Amorim. This introductory plenary session serves as an introduction to ATP-BIO, a major new US-based research center for cryobiology, and celebrates recent honorees of the Society for Cryobiology.4 SubsessionsOPENING REMARKS8:30 AM -8:40 AMSpeakers: Greg M. Fahy, Christiani Amorim INVITED SPEAKER: S1 ATP-BIO, A NEW CENTER USING NANOMEDICINE TO PRESERVE BIOLOGICAL SYSTEMS8:40 AM -9:40 AMSpeakers: John Bischof, Mehmet Toner CRYOFELLOW PRESENTATION: ERIK WOODS9:40 AM -10:15 AMSpeaker: Erik Woods DAYONG GAO YOUNG INVESTIGATOR AWARD, SPONSORED BY GOLDSIM10:15 AM -10:30 AMSpeaker: To be announced live

COFFEE BREAK 10:30 AM -11:00 AMat O'Connell Suite

PLENARY SESSION 2: IN MEMORIAM DAVID PEGG 11:00 AM -1:00 PMat The Ballroom Sponsored By:Sylvatica Biotech Co-Chairs: Michael Taylor and Barry Fuller. David E. Pegg, MD. FRC path. F Soc Cryobiol., a pioneer in cryobiology died on August 3rd 2019 (In Memoriam: Cryobiology 91 (2019) 1-2). Prof. Pegg leaves a rich legacy of contributions to the field of cryobiology. This memorial symposium is a celebration ... Read More6 SubsessionsINVITED SPEAKER: S2 DAVID PEGG’S INSIGHTS INTO FREEZING INJURY IN CELLS, INCLUDING THE ‘UNFROZEN FRACTION’ DEBATE WITH PETER MAZUR11:00 AM -11:20 AMSpeaker: John Armitage INVITED SPEAKER: S3 CRYOBIOLOGY OF MULTICELLULAR SYSTEMS: ROLE OF ICE AVOIDANCE, TOLERANCE, AND PROMOTION IN TISSUE CRYOPRESERVATION11:20 AM -11:40 AMSpeaker: Mike Taylor INVITED SPEAKER: S4 DAVID PEGG AND THE COLD CHAIN FOR ORGANS: A DECADE OF OBJECTIVE AND INSIGHTFUL SCIENCE AT THE DAWN OF CLINICAL TRANSPLANT SERVICES11:40 AM -12:00 PMSpeaker: Barry Fuller INVITED SPEAKER: S5 DAVID PEGG, PIONEER OF ORGAN CRYOPRESERVATION AND ELECTROMAGNETIC HEATING12:00 PM -12:20 PMSpeaker: Brian Wowk INVITED SPEAKER: S6 DAVID PEGG AND THE DEVELOPMENT OF THE SCIENCE OF TISSUE BANKING AND ITS REGULATION IN THE UK12:20 PM -12:40 PMSpeaker: Charles J. Hunt INVITED SPEAKER: S7 HIGH AND LOW SUBZERO ENGINEERING APPROACHES FOR ORGAN PRESERVATION12:40 PM -1:00 PMSpeaker: John Bischof

LUNCH 1:00 PM -2:00 PMat O'Connell Suite

PLENARY SESSION 3: CELEBRATING 50 YEARS OF THE INSTITUTE FOR PROBLEMS OF CRYOBIOLOGY AND CRYOMEDICINE (IPCC) - HISTORY AND FUTURE OUTLOOK 2:00 PM -3:30 PMat The Ballroom Co-Chairs: Yuri Petrenko and Iryna Buriak. This year is the 50th anniversary of the world-recognized Institute for Problems of Cryobiology and Cryomedicine (IPC&C) of the National Academy of Sciences of Ukraine (NASU). In this session, we will recall the history of IPC&C, establishment of co... Read More4 SubsessionsINVITED SPEAKER: INTRODUCTION TO THE IPCC SESSION2:00 PM -2:05 PMSpeaker: Alexander Petrenko INVITED SPEAKER: S8 (V) CONTACTS AND LINKS BETWEEN SLTB, CRYOBIOLOGY AND IPCC. GROWTH AND COLLABORATION TO CHAMPION CRYOBIOLOGY2:05 PM -2:35 PMSpeaker: Brian Grout INVITED SPEAKER: S9 (V) THE IMPACT OF THE UNESCO CHAIR IN RESEARCH COLLABORATIONS2:35 PM -3:00 PMSpeaker: Maria Celeste Robert INVITED SPEAKER: S10 INSTITUTE FOR PROBLEMS OF CRYOBIOLOGY AND CRYOMEDICINE OF THE NAS OF UKRAINE: FUTURE OUTLOOK3:00 PM -3:30 PMSpeaker: Alexander Petrenko

COFFEE BREAK 3:30 PM -4:00 PMat O'Connell Suite

PLENARY SESSION 3 CONT.: CELEBRATING 50 YEARS OF THE IPCC - OVERVIEW OF DISCIPLINES AND CURRENT RESEARCH 4:00 PM -6:20 PMat The Ballroom Co-Chairs: Yuri Petrenko and Iryna Buriak. The exclusivity of IPC&C as a world-known cryobiology research centre lies in the combination of distinct cryobiology disciplines: from fundamental cryobiology to biodiversity preservation, reproductive cryobiology and stem cell cryopreservation. In thi... Read More7 SubsessionsINVITED SPEAKER: S11 FISH SPERM CRYOBANKING IN THE VIEW OF INTERNATIONAL COOPERATION4:00 PM -4:20 PMSpeaker: Borys Dzyuba INVITED SPEAKER: S12 THE CONTRIBUTION OF THE

NIAN SCIENCE TO THE DEVELOPMENT OF HUMAN REPRODUCTIVE CRYOBIOLOGY4:20 PM -4:40 PMSpeaker: Taisiia Yurchuk INVITED SPEAKER: S13 (V) IPC&C NAS OF UKRAINE CONTRIBUTION TO THE IMPLEMENTATION OF THE STATE PROGRAM OF RESEARCH IN ANTARCTICA4:40 PM -5:00 PMSpeaker: Anton Puhovkin INVITED SPEAKER: S14 THEORETICAL APPROACHES IN CRYOBIOLOGY5:00 PM -5:20 PMSpeaker: Olga Gordiyenko INVITED SPEAKER: S15 LOW-TEMPERATURE STABILIZATION OF PROTEINS5:20 PM -5:40 PMSpeaker: Svitlana Rozanova INVITED SPEAKER: S16 THERAPEUTIC EFFECT OF WOUND DRESSING CONTAINING CRYOPRESERVED COMPONENTS5:40 PM -6:00 PMSpeaker: Iryna Buriak INVITED SPEAKER: S17 THE OVERVIEW OF STEM CELL RESEARCH IN THE INSTITUTE FOR PROBLEMS OF CRYOBIOLOGY AND CRYOMEDICINE6:00 PM -6:20 PMSpeaker: Yuri Petrenko

CELEBRATING 50 YEARS OF THE IPCC - RECEPTION 6:30 PM -8:30 PMat O'Connell Suite



__________20.07.2022________

SESSION 1: CRYOPROTECTANTS & ICE FORMATION 1 8:30 AM -10:30 AMat The Ballroom Co-Chairs: Jason Acker and Robert Ben. The creation of new, and optimization of existing, cryoprotectants and cryopreservation strategies are required as conventional cryoprotectants and protocols often fail to provide the high post-thaw recoveries required by emerging applications of the post-thaw ... Read More5 SubsessionsS18 TREE POLLEN DERIVED ICE-NUCLEATING MACROMOLECULES ENABLES CRYOPRESERVATION OF MAMMALIAN CELL MONOLAYERS IN 96-WELL PLATES8:30 AM -8:50 AMSpeaker: Thomas F. Whale S19 (V) NEOTERIC SOLVENTS FOR CRYOPRESERVATION8:50 AM -9:10 AMSpeaker: Miyah Awad S20 INFLUENCE OF ICE FORMATION ON THE DYNAMIC AND THERMODYNAMIC PROPERTIES OF AQUEOUS SOLUTIONS9:10 AM -9:30 AMSpeaker: Jorge Humberto Melillo S21 POLYMER-MEDIATED CRYOPRESERVATION OF BACTERIOPHAGES9:30 AM -9:50 AMSpeaker: Huba L. Marton S22 (V) AMPHIPHILES AS A TOOL OF STUDYING THE STATE OF CELLS UNDER TEMPERATURE-OSMOTIC SHOCK9:50 AM -10:10 AMSpeaker: Olena Chabanenko

SYMPOSIUM 1: FERTILITY PRESERVATION 8:30 AM -10:30 AMat Trinity 1 Co-Chairs: Christiani Amorim and Pierre Comizzoli. There are numerous benign conditions and malignancies threatening fertility in young women, men, and children and therefore fertility preservation options, such as cryopreservation of oocytes, sperm, embryos, or reproductive tissue, should be offere... Read More4 SubsessionsINVITED SPEAKER: S23 THE IMPORTANCE OF OVARIAN TISSUE TRANSPORTATION FOR FERTILITY OUTCOMES8:30 AM -9:00 AMSpeaker: Christiani Amorim INVITED SPEAKER: S24 DRIED FOR THE FUTURE: LONG-TERM STORAGE OF ANIMAL GERMPLASMS AT AMBIENT TEMPERATURES9:00 AM -9:30 AMSpeaker: Pierre Comizzoli INVITED SPEAKER: S25 (V) CRYOPRESERVATION OF IMMATURE TESTICULAR TISSUE AS A FERTILITY PRESERVATION STRATEGY FOR PREPUBERTAL BOYS: CURRENT STRATEGIES9:30 AM -10:00 AMSpeaker: Christine Wyns INVITED SPEAKER: S26 (V) TRANSPORTATION OF HUMAN OVARIAN TISSUE: A SYSTEMATIC REVIEW10:00 AM -10:30 AMSpeaker: Hajra Khattak

SYMPOSIUM 2: CRYOBIOTECHNOLOGY OF EXCEPTIONAL PLANT SPECIES: CHALLENGES AND SUCCESSES 8:30 AM -10:30 AMat Trinity 2 Co-Chairs: Daniel Ballesteros and Raquel Folgado. Exceptional plant species are those that present seeds that are not amenable for conventional seed banking and require cryopreservation for their long term ex situ conservation. The vast diversity of species with varying physiology and the increasing... Read More4 SubsessionsINVITED SPEAKER: S27 ADDRESSING THE NEEDS FOR CONSERVING THREATENED EXCEPTIONAL SPECIES—THE CRITICAL ROLE OF CRYOPRESERVATION8:30 AM -9:00 AMSpeaker: Valerie Pence INVITED SPEAKER: S28 CAN THERMAL FINGERPRINTS OF MEDITERRANEAN TERRESTRIAL ORCHID SEEDS BE USED TO OPTIMIZE EX SITU CONSERVATION?9:00 AM -9:30 AMSpeaker: Sara Magrini INVITED SPEAKER: S29 MODIFICATION OF STRESS FACTORS AND ICE CRYSTALLIZATION BEHAVIOUR FOR SUCCESSFUL CRYOPRESERVATION OF DUCKWEED SPECIES9:30 AM -10:00 AMSpeaker: Manuela Nagel INVITED SPEAKER: S30 CRYOBIOTECHNOLOGY FOR THE CONSERVATION OF BRYOPHYTES10:00 AM -10:30 AMSpeaker: Daniel Ballesteros

COFFEE BREAK 10:30 AM -11:00 AMat O'Connell Suite

SESSION 2: PRACTICAL CONCERNS AND CONSIDERATIONS IN BIOBANKING 11:00 AM -1:00 PMat The Ballroom Co-Chairs: Charles J. Hunt and Glyn Stacey. This session on biobanking will consider some of the key practical challenges for biobank maintenance including microbial contamination, thermal cycling and the suitability of different cryocontainers. It will also address the exceptional challenge of sust... Read More6 SubsessionsS31 (V) MANAGEMENT OF GAMETE AND EMBRYO CRYOBANKS DURING MILITARY OPERATIONS11:00 AM -11:20 AMSpeaker: Maryna Petrushko INVITED SPEAKER: S32 THE GENERATION OF THERMAL TRANSIENTS DURING ROUTINE ACCESS TO CRYOVIALS STORED IN VAPOUR-PHASE LIQUID NITROGEN – IMPLICATIONS FOR BIOBANKS11:20 AM -11:40 AMSpeaker: Charles J. Hunt INVITED SPEAKER: S33 MICROBIAL CONTAMINATION OF BIO-STORAGE TANKS CAN RISK REPRODUCIBILITY OF DATA AND ENGENDER PATHOGEN TRANSFER11:40 AM -12:00 PMSpeaker: Glyn Stacey S34 ENHANCING QUALITY BIOBANKING THROUGH EDUCATION12:00 PM -12:20 PMSpeaker: Alison Parry-Jones S35 A CLOSER LOOK ON TIGHTNESS ISSUES OF CRYOVIALS AND STRAWS IN BIOBANKING12:20 PM -12:40 PMSpeaker: René Kretschmer S36 IS THE OCCURRENCE OF MICROORGANISM LIQUID NITROGEN STORAGE TANKS A CHALLENGE FOR CRYOBANKING?12:40 PM -1:00 PMSpeaker: Felizitas Bajerski

SESSION 3: RECENT ADVANCES IN THE CRYOPRESERVATION OF OOCYTES AND OVARIAN TISSUE 11:00 AM -1:00 PMat Trinity 1 Co-Chairs: Roland Fleck and Ramon Risco. This session will cover oocyte and ovarian cell and tissue cryobiology of human, agricultural, and laboratory animals. The speakers will provide brief information about the current status of representative species and their research findings.6 SubsessionsS37 THE EFFECTS OF ERYTHROPOIETIN ON ISCHEMIA-REPERFUSION WHEN ADMINISTERED BEFORE AND AFTER OVARIAN TISSUE TRANSPLANTATION IN MICE11:00 AM -11:20 AMSpeaker: Fernanda Paulini S38 EXOGENOUS MELATONIN AMELIORATES THE NEGETIVE EFFECT OF OSMOTIC STRESS IN HUMAN AND BOVINE OVARIAN STROMAL CELLS11:20 AM -11:40 AMSpeaker: Ebrahim Asadi S39 VITRIFICATION OF BOVINE OVARIAN TISSUE AS A MODEL FOR HUMAN: THE EFFICIENCY OF NON-PERMEATING CPAS AND CLOSED SYSTEM11:40 AM -12:00 PMSpeaker: Atefeh Najafi S40 INCREASE IN VITRO MATURATION RATE OF VITRIFIED MOUSE GV OOCYTES VIA NOVEL XYLOMANNAN COATED IRON OXIDE NANOCOMPOSITE12:00 PM -12:20 PMSpeaker: Rouhollah Fathi S41 (V) CRYOPRESERVATION OF HUMAN CUMULUS CELLS BY 3-STEP SLOW FREEZING METHOD FOR OOCYTE IN VITRO MATURATION SYSTEM12:20 PM -12:40 PMSpeaker: Taisiia Yurchuk S42 (V) A MICROFLUIDIC APPROACH FOR SIMULTANEOUS AND NONDESTRUCTIVE INVESTIGATION OF OOCYTE MEMBRANE PERMEABILITY12:40 PM -1:00 PMSpeaker: Zhongrong Chen

SESSION 4: RECENT ADVANCES IN PLANT CRYOBIOTECHNOLOGY FOR PRESERVATION OF AGRICULTURAL AND HORTICULTURAL SPECIES 11:00 AM -12:40 PMat Trinity 2 Co-Chairs: Daniel Ballesteros and Manuela Nagel. Many crops and ornamental plants are propagated clonally, so virus-free meristems in vegetative tissues (instead of seeds) are those preferably stored for preservation. This is made for the short-medium term in living or in vitro collections. However,... Read More5 SubsessionsS43 IMPROVEMENT OF RECOVERY CYCLE OF CRYOPRESERVED POTATO SHOOT TIPS - SMALL CHANGES, BUT HUGE IMPACT11:00 AM -11:20 AMSpeaker: Rainer Vollmer S44 (V) COLD PRETREATMENT FOR CRYOPRESERVATION OF AN ENDANGERED AUSTRALIAN NATIVE PLANT SPECIES GOSSIA FRAGRANTISSIMA11:20 AM -11:40 AMSpeaker: Jingyin Bao S45 CRYOBIOTECHNOLOGICAL APPROACHES TO ELIMINATE SUGARCANE MOSAIC VIRUS (SCMV) IN SUGARCANE (Saccharum spp. L.) INFECTED PLANTS11:40 AM -12:00 PMSpeaker: María Teresa González-Arnao S46 A MULTI-MODAL ANALYSIS OF MENTHA X PIPERITA SHOOT TIP RESPONSE TO CRYOPROTECTIVE AGENTS12:00 PM -12:20 PMSpeaker: Heidi D. Kreckel S47 (V) CRYOPRESERVATION OF CUCURBITACEAE SPECIES POLLEN FOR EXPLORING BREEDING POSSIBILITIES12:20 PM -12:40 PMSpeaker: Rajasekharan Pe

SESSION 5: LYOPHILIZATION FOR PRESERVATION 12:40 PM -1:00 PMat Trinity 2 Co-Chairs: Daniel Ballesteros and Manuela Nagel. Lyophilization is commonly used in food manufacturing and preservation. This presentation aims to clarify the effect of unfrozen water content on the physical quality of the freeze-dried food model.1 SubsessionS48 (V) EFFECT OF UNFROZEN WATER CONTENT ON THE COLLAPSE OF FREEZE-DRIED FOOD MODEL12:40 PM -1:00 PMSpeaker: Sukritta Anantawittayanon

LUNCH 1:00 PM -1:50 PMat O'Connell Suite

SESSION 6: CRYOPROTECTANTS & ICE FORMATION 2 1:50 PM -3:30 PMat The Ballroom Co-Chairs: Michael Taylor and Robert Ben. The creation of new, and optimization of existing, cryoprotectants and cryopreservation strategies are required as conventional cryoprotectants and protocols often fail to provide the high post-thaw recoveries required by emerging applications of the post-th... Read More5 SubsessionsS155 IMPROVEMENT OF SLOW FREEZING PROTOCOL FOR ADULT CAENORHABDITIS ELEGANS1:50 PM -2:10 PMSpeaker: Laura Encabo S49 PROBING INTERACTIONS BETWEEN SMALL-MOLECULE ICE RECRYSTALLIZATION INHIBITORS (IRIS) AND WATER MOLECULES USING 1H NMR2:10 PM -2:30 PMSpeaker: Leah E. McMunn S50 IMPACT OF FREEZING METHOD ON PRESERVATION OUTCOMES2:30 PM -2:50 PMSpeaker: Quinn Osgood S51 (V) HOW DOES DIMETHYL SULFOXIDE MODULATE GLYCOLYSIS AND GLUCONEOGENESIS PATHWAYS?2:50 PM -3:10 PMSpeaker: Neda Ghousifam S52 (V) SCREENING AND OPTIMIZATION OF CRYOPROTECTIVE AGENTS (CPAS) FOR ELECTROMAGNETIC HEATING OF CRYOPRESERVED BIOMATERIALS3:10 PM -3:30 PMSpeaker: Ziyuan Wang

SYMPOSIUM 3: ORGAN PRESERVATION FOR TRANSPLANTATION 1:50 PM -3:30 PMat Trinity 1 Co-Chairs: Shannon Tessier (V) and Rohil Jain. Every year about 154,000 organ transplants take place in the world. On the other hand, the World Health Organization (WHO) estimates that the current availability of organs meets only about 10% of the global demand. Recent research to address the gap fo... Read More4 SubsessionsINVITED SPEAKER: S53 CLINICAL AND POLICY IMPLICATIONS OF ORGAN PRESERVATION1:50 PM -2:15 PMSpeaker: Erik Finger INVITED SPEAKER: S54 EX VIVO AND IN SITU OXYGENATED CADAVER ORGAN CARE IN MURINE TEMPERATURE-CONTROLLED ORGAN-PRESERVING MACHINE AND ECMO2:15 PM -2:40 PMSpeaker: Nodir Madrahimov INVITED SPEAKER: S55 (V) CURRENT PERSPECTIVE AND ROLE OF BIOLOGICAL ANTIFREEZE PROTEINS (AFP) IN SUCCESSFUL ORGAN CRYOPRESERVATION2:40 PM -3:05 PMSpeaker: Muhammad Shuaib Khan INVITED SPEAKER: S56 INCREASED VENOUS PRESSURE: ANOTHER BEAUTIFUL IDEA BITES THE DUST3:05 PM -3:30 PMSpeaker: Greg M. Fahy

COFFEE BREAK 3:30 PM -4:00 PMat O'Connell Suite

SESSION 7: RECENT ADVANCES IN CELLULAR THERAPY 4:00 PM -6:20 PMat The Ballroom Co-Chairs: Robert Ben and Glyn Stacey. Cell therapy involves a complex supply chain and preservation is a critical element of that supply chain. Speakers in this session will address topics ranging from the challenges of developing cell therapies, implementing cell therapy in a manufacturing setting... Read More7 SubsessionsS57 SMALL MOLECULE ICE RECRYSTALLIZATION INHIBITORS DRAMATICALLY IMPROVE THE CRYOPRESERVATION OF CELLULAR THERAPY PRODUCTS4:00 PM -4:20 PMSpeaker: Robert Ben S58 EXPLORING HIGH CELL CONCENTRATION CRYOPRESERVATION FOR DELIVERY OF ENGINEERED T CELL PRODUCTS4:20 PM -4:40 PMSpeaker: Tracey R. Turner S59 DROPLET VITRIFICATION OF GRANULOCYTES: A PROOF-OF-CONCEPT STUDY4:40 PM -5:00 PMSpeaker: Nishaka William S60 CRYOPRESERVATION TECHNIQUES IN MANUFACTURING,TRANSPORT AND STORAGE OF CAR-T THERAPY PRODUCTS -INITIAL EXPERIENCE AND PROPOSALS FOR FUTURE IMPROVEMENTS5:00 PM -5:20 PMSpeaker: Glyn Stacey S61 (V) CRYOPRESERVATION OF MESENCHYMAL STEM CELL-BASED 3D CONSTRUCTS5:20 PM -5:40 PMSpeaker: Olena Rogulska S62 (V) EFFECT OF COOLING RATE ON THE INCIDENCE OF APOPTOSIS IN CRYOPRESERVED T CELLS5:40 PM -6:00 PMSpeaker: Jens Karlsson S63 (V) ESTABLISHING AN OPTIMISED PROTOCOL FOR CRYOPRESERVATION OF HEPATOCYTE MICROBEADS FOR CLINICAL USE6:00 PM -6:20 PMSpeaker: Shaikha Almazrouei

SYMPOSIUM 3: ORGAN PRESERVATION FOR TRANSPLANTATION (CONTINUED) 4:00 PM -6:00 PMat Trinity 1 Co-Chairs: Shannon Tessier (V) and Rohil Jain.6 SubsessionsINVITED SPEAKER: S64 24-HOUR SUPERCOOLING PRESERVATION OF VASCULARIZED COMPOSITE ALLOGRAFTS IN RATS: A PROOF-OF-CONCEPT STUDY4:00 PM -4:20 PMSpeaker: Irina Filz von Reiterdank INVITED SPEAKER: S65 (V) TOOLS FOR HEART PRESERVATION RESEARCH4:20 PM -4:40 PMSpeaker: Manuela Lopera Higuita INVITED SPEAKER: S66 (V) BIOLOGICAL CHARACTERIZATION OF VITRIFIED PANCREATIC ISLETS AND TRANSPLANTATION4:40 PM -5:00 PMSpeaker: Joseph Sushil Rao INVITED SPEAKER: S67 (V) VITRIFICATION AND NANOWARMING OF KIDNEYS5:00 PM -5:20 PMSpeaker: Zonghu Han INVITED SPEAKER: S68 (V) VITRIFICATION AND REWARMING OF MAGNETIC NANOPARTICLE-LOADED RAT HEARTS5:20 PM -5:40 PMSpeaker: Bat-Erdene Namsrai INVITED SPEAKER: S69 (V) ADVANCING ORGAN CRYOPRESERVATION THROUGH SCALABLE POLYMER COATING OF IRON OXIDE NANOPARTICLES5:40 PM -6:00 PMSpeaker: Jacqueline Pasek-allen

SESSION 8: MODELING TO OPTIMIZE CRYOPRESERVATION 4:00 PM -6:20 PMat Trinity 2 Co-Chairs: James Benson and Roman Bauer. Cryopreservation exposes cells and tissues to changes in temperature and solution composition that can cause damage by a variety of mechanisms, including osmotic damage and cryoprotectant toxicity. This session will focus on mathematical modeling approaches f... Read More7 SubsessionsS70 COMPUTATIONAL MODELLING AND OPTIMISATION OF SLOW COOLING PROFILES FOR THE CRYOPRESERVATION OF CELLS IN SUSPENSION4:00 PM -4:20 PMSpeaker: Roman Bauer S71 (V) SUPERCOOLED BIOPRESERVATION PROTOCOL DESIGN USING PROBABILISTIC SAFETY ANALYSIS4:20 PM -4:40 PMSpeaker: Anthony N. Consiglio S72 (V) hMSCs IN CONTACT WITH Me2SO FOR CRYOPRESERVATION: EXPERIMENTS AND MODELLING OF OSMOTIC INJURY AND CYTOTOXIC EFFECT4:40 PM -5:00 PMSpeaker: Gabriele Traversari S73 OBTAINING TEMPERATURE DEPENDENCE OF CELL MEMBRANE PERMEABILITY PARAMETERS USING NON-IDEAL THERMODYNAMIC ASSUMPTIONS TO MATHEMATICALLY MODEL REAL CRYOPRESERVATION PROTOCOLS5:00 PM -5:20 PMSpeaker: Faranak Yadegari S74 (V) AN AGENT BASED MODEL OF INTRACELLULAR ICE FORMATION AND PROPAGATION IN SMALL TISSUES5:20 PM -5:40 PMSpeaker: Fatemeh Amiri S75 WITHDRAWN5:40 PM -5:40 PM S76 (V) ELECTROMAGNETIC REWARMING FOR CRYOPRESERVATION: A NUMERICAL COMPARISON BETWEEN MULTI-MODE AND SINGLE-MODE ELECTROMAGNETIC CAVITY5:40 PM -6:00 PMSpeaker: Ruidong Ma

IN PERSON POSTER SESSION 6:30 PM -8:30 PMat O'Connell Suite Browse in person posters in the O'Connell Suite. Browse virtual and in person posters at your leisure in Whova.


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SESSION 10: COMPARATIVE SPERM CRYOPRESERVATION 8:00 AM -10:20 AMat Trinity 1 Co-Chairs: Ido Braslavsky and Erik Woods. This session will cover human, agricultural, wild, and laboratory species. Speakers will provide brief summaries about the current status of representative species and their research findings.7 SubsessionsS84 COMPREHENSIVE LIPIDOMICS USING TARGETED MRM LCMS METHOD IN BOVINE SPERM8:00 AM -8:20 AMSpeaker: Muhammad Kaleem Sarwar S85 DIMETHYL SULFOXIDE AND QUERCETIN PROLONGED STORAGE PERIOD OF COLD-STORED RAT SPERM8:20 AM -8:40 AMSpeaker: Katsuma Yamaga (V) S86 CREATING MULTIPLE GENERATIONS OF A THREATENED AMPHIBIAN SPECIES USING ASSISTED REPRODUCTIVE TECHNOLOGIES8:40 AM -9:00 AMSpeaker: Isabella J. Burger S87 THE PHENOMENON OF ZERO SURVIVAL OF SPERM CELLS CRYOPRESERVED IN NANOLITER VOLUMES9:00 AM -9:20 AMSpeaker: Bat-sheva Galmidi S88 HYDROPHOBIC SOOT NANOPARTICLES APPLICABLE TO CRYOBIOLOGY AND REPRODUCTIVE MEDICINE AS A FUNCTIONAL ACTIVATOR OF HUMAN SPERMATOZOA9:20 AM -9:40 AMSpeaker: Karekin Esmeryan (V) S89 GENOME-WIDE ASSOCIATION STUDY OF CRYOPRESERVED SEMEN QUALITY TRAITS IN HOLSTEIN-FRIESIAN BULLS9:40 AM -10:00 AMSpeaker: Laura Abril-Parreño (V) S90 EVALUATION OF THE METHLATION PATTERN OF IMPRINTING GENES IN HUMAN SPERM DURING RAPID FREEZING VERSUS VITRIFICATION10:00 AM -10:20 AMSpeaker: Nahid Khosronezhad

SESSION 9: RECENT ADVANCES IN VITRIFICATION 8:30 AM -10:30 AMat The Ballroom Co-Chairs: Kelvin Brockbank and Greg Fahy. We open this session with a biomaterials perspective on vitrification followed by presentations on control and assessment of vitrification. It will be informative to see the strategies employed in three presentations on vitrification of 3D tissue constructs... Read More7 SubsessionsS77 A MATERIALS SCIENCE VIEW OF CRYOPRESERVATION VIA GLASS FORMATION8:30 AM -8:50 AMSpeaker: William Brower S78 INVITED SPEAKER: VITRIFICATION PROCESS CONTROL BY DSC8:50 AM -9:10 AMSpeaker: Milos Faltus S79 ASSESSMENT OF VITRIFICATION STATE OF SUPERFLASH FROZEN DROPLETS BASED ON FLUORESCENCE SELF-QUENCHING9:10 AM -9:30 AMSpeaker: Hiroki Watanabe S80 VITRIFICATION OF BIO-ENGINEERED EPITHELIAL CONSTRUCTS9:30 AM -9:50 AMSpeaker: Kelvin G.M. Brockbank S81 (V) VITRIFICATION OF TISSUE ENGINEERED CONSTRUCTS BASED ON MESENCHYMAL STROMAL CELLS9:50 AM -10:10 AMSpeaker: Natalia Trufanova S82 moved to Novel Tools & Technologies 210:10 AM -10:10 AM S83 CRYOPRESERVATION OF IPSCS DERIVED NEURAL 3D CELL MODELS AS A FUNDAMENTAL KEY TECHNOLOGY IN BIOTECHNOLOGY AND BIOMEDICINE10:10 AM -10:30 AMSpeaker: Saskia Altmaier

COFFEE BREAK 10:20 AM -11:00 AMat O'Connell Suite

SYMPOSIUM 4: NOVEL TECHNOLOGIES AND THE FUTURE OF CRYOPRESERVATION 11:00 AM -1:05 PMat The Ballroom Co-Chairs: Roland Fleck and Peter Kilbride. The development of novel tools and technologies can benefit cryobiological study and applications by proposing new solutions to existing challenges. In this session speakers will discuss a wide variety of novel tools and technologies for cryopreservation a... Read More5 SubsessionsINVITED SPEAKER: S91 CRYOPRESERVATION IN BIOMEDICAL ENGINEERING11:00 AM -11:25 AMSpeaker: Birgit Glasmacher INVITED SPEAKER: S92 A CRITICAL TRANSFER TEMPERATURE FROM SLOW COOLING TO CRYOGENIC STORAGE FOR OPTIMAL RECOVERY ACROSS A RANGE OF CRYOPRESERVED MAMMALIAN CELLS11:25 AM -11:50 AMSpeaker: Julie Meneghel INVITED SPEAKER: S93 DEVELOPING LN2‐FREE SHIPPING FOR CRYOPRESERVED CELL THERAPIES11:50 AM -12:15 PMSpeaker: Peter Kilbride INVITED SPEAKER: S94 CRYOBIOLOGY CAN SHAPE THE FUTURE OF ENERGY EFFICIENCY12:15 PM -12:40 PMSpeaker: Elias Anastassopoulos INVITED SPEAKER: S95 CURRENT AND FUTURE POTENTIAL OF ELECTRON MICROSCOPY IN THE HIGH-RESOLUTION 3D STUDY OF CELLS, TISSUES AND IN SITU MACROMOLECULES12:40 PM -1:05 PMSpeaker: Roland Fleck

SESSION 11: CRYOPRESERVATION APPLIED TO AQUATIC ORGANISMS 11:00 AM -1:00 PMat Trinity 1 Co-Chair: Estefania Paredes and Huiping Yang. This session will discuss advances in cryobiology specific to aquatic organisms (marine and freshwater) for conservation, research and Aquaculture.6 SubsessionsS96 CRYOPRESERVING MOLLUSKS SINCE 197011:00 AM -11:20 AMSpeaker: Estefania Paredes S97 APPLICATION OF GERMPLASM CRYOPRESERVATION TO A BREEDING PROGRAM OF THE EASTERN OYSTER CRASSOSTREA VIRGINICA11:20 AM -11:40 AMSpeaker: Huiping Yang S98 CRYOPRESERVATION DAMAGE ON SEA URCHIN OOCYTES ANALYZED BY ELECTRON MICROSCOPY11:40 AM -12:00 PMSpeaker: Sara Campos S99 MODULATION OF STRESS FACTORS FOR CRYOPRESERVATION OF P. LIVIDUS LARVAE12:00 PM -12:20 PMSpeaker: Alba Lago S100 MOTILE SPERM FRACTION SEPARATION FROM CRYOPRESERVED SAMPLES IN TAXONOMICALLY DISTANT FISHES12:20 PM -12:40 PMSpeaker: Borys Dzyuba S101 PROTOCOL FOR COMMON CARP SPERM CRYOPRESERVATION FOR AQUACULTURE-SCALE ARTIFICIAL REPRODUCTION12:40 PM -1:00 PMSpeaker: Anatolii Sotnikov

LUNCH 1:00 PM -1:50 PMat O'Connell Suite

COMPETITION: CRYSTAL AWARD 1:50 PM -3:30 PMat The Ballroom Co-Chairs: Estefania Paredes and Barry Fuller. Join the cryobiology leaders of tomorrow as they compete to win the Peter. L Steponkus Crystal Award for the best student oral presentation. The award carries a $1,000 honorarium.5 SubsessionsS102 CELL AND NUCLEAR OSMOTIC CHARACTERISTICS OF ADHERENT HEPG2 CELLS DETECTED BY USING CONFOCAL MICROSCOPY AND 3D RECONSTRUCTION: IMPLICATIONS FOR CRYOPRESERVATION1:50 PM -2:10 PMSpeaker: Iqra Azam S103 IDENTIFYING THE KEY STRUCTRUAL ATTRIBUTES NESSESSARY FOR IRI ACTIVITY IN ARYL GLUCONAMIDES2:10 PM -2:30 PMSpeaker: Marcus F. Diamante S104 (V) CHARACTERIZING A NOVEL ROTATING COPPER PLATE APPARATUS FOR DROPLET VITRIFICATION2:30 PM -2:50 PMSpeaker: Parth K Patel S105 ENHANCED CONTROL OVER ICE NUCLEATION STOCHASTICITY USING A CARBOHYDRATE POLYMER CRYOPROTECTANT2:50 PM -3:10 PMSpeaker: Bruno Guerreiro S106 STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF ANTIFREEZE PROTEIN APAFP7523:10 PM -3:30 PMSpeaker: Logan Brown

SESSION 12: LEGAL AND ETHICAL CONSIDERATIONS IN CRYOBIOLOGY 2:10 PM -2:30 PMat Trinity 1 Co-Chairs: Charles J. Hunt and Erik Woods. Advances in technology and long term cryopreservation of reproductive cells and tissues can raise legal and ethical questions which merit serious consideration. This session will explore ethics in relation to organ printing and long-term embryo storage.2 SubsessionsS107 WITHDRAWN2:10 PM -2:10 PM S108 WHEN DESIRABLE EMBRYOS ARE CRYOPRESERVED LONG AFTER THEY ARE DESIRABLE2:10 PM -2:30 PMSpeaker: Daniel Kelly

SESSION 13: COMPARATIVE EMBRYO CRYOPRESERVATION 2:30 PM -3:30 PMat Trinity 1 Co-Chairs: Teresa Mogas and Vitalii Mutsenko. This session will cover agricultural and laboratory species. Speakers will provide brief summaries about the current status of representative species and their research findings.3 SubsessionsS109 (V) CRYOPRESERVATION OF ANOPHELES STEPHENSI MOSQUITOES2:30 PM -2:50 PMSpeaker: Eric James S110 THE EFFECTS OF CRYOPROTECTANT, TEMPERATURE AND LENGHT OF IN VITRO CULTURE IN THE PERMEABILITY OF IN VITRO PRODUCED BOVINE EMBRYOS: TOWARDS AN IN SILICO DESIGNED VITRIFICATION/WARMING PROTOCOL2:50 PM -3:10 PMSpeaker: Iris Martínez-Rodero S111 (V) VITRIFICATION OF ONE-CELL RAT EMBRYOS IN CRYOTUBES BY SMALL-VOLUME VITRIFICATION AND RAPID WARMING3:10 PM -3:30 PMSpeaker: Shinsuke Seki

COFFEE BREAK 3:30 PM -4:00 PMat O'Connell Suite

SESSION 14: TOOLS AND TECHNOLOGIES FOR CRYOPRESERVATION AND CRYOPRESERVATION 1 4:00 PM -6:00 PMat The Ballroom Co-Chairs: Birgit Glasmacher and Peter Kilbride. The development of novel tools and technologies can benefit cryobiological study and applications by proposing new solutions to existing challenges. In this session speakers will discuss a wide variety of novel tools and technologies for cryopreservat... Read More6 SubsessionsS112 RECENT ADVANCES IN 3D CRYOPRINTING4:00 PM -4:20 PMSpeaker: Linnea Warburton S113 REVOLUTIONISING CELL BANKING WITH MONOLAYER CRYOPRESERVATION: A NEW PLATFORM FOR DRUG SCREENING4:20 PM -4:40 PMSpeaker: Ruben Tomas S114 UPSCALING CRYOPRESERVATION PROTOCOLS FROM MEDIUM TO LARGE SCALE, SUITABLE FOR CLINICAL CELL THERAPY PRODUCTION4:40 PM -5:00 PMSpeaker: Thomas Brookshaw S115 INVESTIGATION OF CRYOPROTECTANT THERMOPHYSICAL PROPERTIES IN THE FAST COOLING CRYOPRESERVATION BY DSC TECHNIQUE5:00 PM -5:20 PMSpeaker: Mohammd Amini S116 MOLECULAR MOBILITY AND CONCENTRATION OF PLANT CELLS INTRACELLULAR COMPARTMENTS, AS DERIVED FROM ICE CRYSTAL SIZE MEASURED ON CRYO-SEM MICROGRAPHS5:20 PM -5:40 PMSpeaker: Antonio D. Molina-Garcia S117 CHANGES OF SAMPLE TEMPERATURE INSIDE A CRYO STORAGE ROBOT5:40 PM -6:00 PMSpeaker: Birgit Glasmacher

SESSION 15: LESSONS FROM NATURE: PLANT AND ANIMAL ADAPTATIONS TO COLD 4:00 PM -6:00 PMat Trinity 1 Co-Chairs: Ido Braslavsky and Alexandra Stolzing. The solution to winter survival for many species is hibernation. Recent advances in technologies, new ideas about regulatory mechanisms, and broader studies of diverse species are rapidly expanding our knowledge about plant and animal life in the col... Read More6 SubsessionsS118 CRYOPROTECTIVE ABILITIES OF BARLEY SEED PROTEINS4:00 PM -4:20 PMSpeaker: Prakriti Kashyap S119 AN INSECT ANTIFREEZE PROTEIN FROM ANATOLICA POLITA ENHANCES THE CRYOPROTECTION OF MAMMALIAN CELLS4:20 PM -4:40 PMSpeaker: Krisztina Varga S120 (V) IDENTIFICATION OF COLD SHOCK PROTEINS IN HELIX POMATIA SNAILS: THE PUTATIVE ROLE IN OVERWINTERING4:40 PM -5:00 PMSpeaker: Paulina A. Idczak S121 (V) CRYOPRESERVATION OF DIVERSE SYMBIODINIACEAE DINOFLAGELLATES: ASSESSMENT OF THEIR FATTY ACID PROFILES IN RESPONSE TO INCREASED SALINITY TREATMENTS5:00 PM -5:20 PMSpeaker: Joseph K. Kihika S122 (V) ACTIVATION OF THE HIPPO PATHWAY IN RANA SYLVATICA: YAPPING STOPS IN RESPONSE TO ANOXIA5:20 PM -5:40 PMSpeaker: Aakriti Gupta S123 (V) PSYCHROPHILES AND PSYCHROTOLERANTS: SUSTAINABLE SOURCE OF BIO-BASED ECONOMY5:40 PM -6:00 PMSpeaker: Mohammed Kuddus

SOCIETY FOR CRYOBIOLOGY ANNUAL GENERAL MEETING 6:15 PM -7:15 PMat The Ballroom


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SESSION 16: NOVEL TOOLS AND TECHNOLOGIES FOR CRYOPRESERVATION AND CRYOPRESERVATION 2 8:10 AM -10:30 AMat The Ballroom Co-Chairs: Roland Fleck and Peter Kilbride. The development of novel tools and technologies can benefit cryobiological study and applications by proposing new solutions to existing challenges. In this session speakers will discuss a wide variety of novel tools and technologies for cryopreservation a... Read More7 SubsessionsS82 EXPERIMENTAL PROOF OF ICE RECRISTALLIZATION INHIBITION ON THE C. ELEGANS MODEL BASED ON A SCALABLE WARMING APPROACH BY MEANS OF HIGH INTENSITY FOCUSED ULTRASOUND8:10 AM -8:30 AMSpeaker: Enrique Alcala S124 PHYSICAL DEMONSTRATION OF VITRIFICATION OF LITER SCALE CPA SYSTEMS AND CHARACTERIZATION OF 120KW RF COIL FOR NANOWARMING OF LITER SCALE SYSTEMS8:30 AM -8:50 AMSpeaker: Lakshya Gangwar S125 DEEP EUTECTIC SOLVENTS FOR CRYOPRESERVATION8:50 AM -9:10 AMSpeaker: Saffron Bryant S126 QUANTITATIVE ASSESSMENT OF INTRA- AND EXTRACELLULAR CRYOPROTECTANT CONCENTRATIONS DURING FREEZING AND THAWING BY RAMAN SPECTROSCOPY MICROSCOPY9:10 AM -9:30 AMSpeaker: Taijie Zhan S127 WITHDRAWN9:30 AM -9:50 AM S128 (V) AN AUTOMATIC AND CLOSED SYSTEM FOR RAPID REWARMING OF LARGE VOLUME OF CELL CULTURE MEDIA AND CRYOPRESERVED CELL SUSPENSION9:50 AM -10:10 AMSpeaker: Nanye Du S129 (V) VISUALIZING PVS2 COMPONENT PERMEATION INTO ORYZA SATIVA CALLUS CELLS BY COHERENT RAMAN SCATTERING MICROSCOPY10:10 AM -10:30 AMSpeaker: Fionna M.D. Samuels

SYMPOSIUM 5: ADVANCES IN THE CRYOPRESERVATION OF CONVENTIONAL BLOOD PRODUCTS 8:30 AM -10:30 AMat Trinity 1 Co-Chairs: Jason Acker and Olga Mykhailova. This session will provide a review of the current state of clinical blood cell preservation with a focus on the scientific and technical advances being made to improve the storage of this cell therapy. The speakers will describe and discuss the role that c... Read More5 SubsessionsINVITED SPEAKER: S130 DONOR-DEPENDENT VARIABILITY OF OSMOTIC CHARACTERISTICS OF RED BLOOD CELLS OF DIFFERENT SENESCENCE LEVELS8:30 AM -8:55 AMSpeaker: Olga Mykhailova INVITED SPEAKER: S131 DEVELOPING AN OPTIMIZED PRESERVATION SOLUTION FOR THE SUPERCOOLED STORAGE OF RED BLOOD CELLS: A COMPARISON OF TWO EXTRACELLULAR ADDITIVES8:55 AM -9:20 AMSpeaker: Nishaka William INVITED SPEAKER: S132 (V) PRE-DEHYDRATION BY TREHALOSE ENABLES CRYOPRESERVATION OF HUMAN RBCs WITH UNUSUALLY LOW CONCENTRATIONS OF GLYCEROL9:20 AM -9:50 AMSpeaker: Gang Zhao S133 PERIPHERAL BLOOD MONONUCLEAR CELLS: CHALLENGES IN COLLECTION, PROCESSING AND STORAGE OF PBMCS IN A BIOREPOSITORY9:50 AM -10:10 AMSpeaker: Robyn Osborne S134 (V) CRYOGENIC ENRICHMENT OF MALARIA PARASITES FROM WHOLE BLOOD10:10 AM -10:30 AMSpeaker: Rebecca D. Sandlin

COFFEE BREAK 10:30 AM -11:00 AMat O'Connell Suite

SYMPOSIUM 6: RECENT ADVANCES IN CELL THERAPEUTICS 11:00 AM -1:05 PMat The Ballroom Co-Chairs: Charles J. Hunt and Yuri Petrenko. Cryopreservation and cryobanking are the key steps in the manufacturing of advanced cell therapeutics. Aiming to provide wide and fast availability of the cells for therapy, the challenge is to develop non-toxic, clinically-relevant approaches, which wil... Read More5 SubsessionsINVITED SPEAKER: S135 (V) BIOBANKING IN CURRENT AND FUTURE CELL THERAPIES11:00 AM -11:25 AMSpeaker: Denys Pogozhykh INVITED SPEAKER: S136 A MULTINATIONAL TERMIS CONSENSUS ON THE DEVELOPMENT OF PRESERVED CELL-BASED MEDICINES11:25 AM -11:50 AMSpeaker: Iryna Buriak INVITED SPEAKER: S137 ANTI-FREEZE PROTEINS IN MESENCHYMAL STEM CELL CRYOPRESERVATION11:50 AM -12:15 PMSpeaker: Alexandra Stolzing INVITED SPEAKER: S138 (V) SUSPENDED ANIMATION OF COMPLEX SYSTEMS FOR RESEARCH AND CLINIC12:15 PM -12:40 PMSpeaker: Shannon Tessier INVITED SPEAKER: S139 (V) Me2SO-FREE PRESERVATION OF iPS CELLS AND iPS-DERIVED SENSORY NEURONS12:40 PM -1:05 PMSpeaker: Allison Hubel

SESSION 17: RECENT ADVANCES IN CELL & TISSUE PRESERVATION 11:00 AM -1:00 PMat Trinity 1 Co-Chairs: Kelvin Brockbank and Erik Woods. This session brings together those involved in preservation research and clinical banking across range of cells and tissues.6 SubsessionsS140 ICE CONTROL DURING CRYOPRESERVATION OF HEART VALVES AND MAINTENANCE OF POST-WARMING CELL VIABILITY11:00 AM -11:20 AMSpeaker: Kelvin G.M. Brockbank S141 CRYOPRESERVATION AND POST-THAW CHARACTERIZATION OF DISSOCIATED HUMAN ISLET CELLS11:20 AM -11:40 AMSpeaker: Leah A Marquez-Curtis S142 FROM CELL TO TISSUE: HYDROGEL ENCAPSULATION BRIDGES THE GAP OF ISLET CRYOPRESERVATION11:40 AM -12:00 PMSpeaker: Yue Cheng S143 (V) CRYOPRESERVATION OF COMPOSITE SCAFFOLDS CONTAINING MAGNETIC NANOPARTICLES FOR TISSUE ENGINEERING12:00 PM -12:20 PMSpeaker: Sara Leal Marin S144 (V) NANOWARMING OF LARGE PORCINE OSTEOCHONDRAL TISSUES: IN VITRO AND SIMULATION STUDIES12:20 PM -12:40 PMSpeaker: Shangping Wang S145 (V) CRYOPRESERVATION OF H9C2 CELLS IN SUSPENSION12:40 PM -1:00 PMSpeaker: Elham Ashrafi

LUNCH 1:00 PM -2:00 PMat O'Connell Suite

AWARD SESSION 2:00 PM -3:15 PMat The Ballroom Co-Chairs: Janet Elliott (V) and Greg Fahy. Join us to find out the winner of the 2021 Arthur W. Rowe Cryobiology Best Paper, followed by virtual presentation of the Luyet CryoFellow medal to Prof. Tiantian Zhang.2 SubsessionsARTHUR W. ROWE BEST PAPER AWARD2:00 PM -2:30 PMSpeaker: To be announced live CRYOFELLOW AWARD: TIANTIAN ZHANG2:30 PM -3:15 PMSpeaker: Tiantian Zhang

SESSION 18: RECENT ADVANCES IN PLANT CRYOBIOTECHNOLOGY FOR PRESERVATION OF WILD SPECIES 2:00 PM -3:20 PMat Trinity 1 Co-Chairs: Daniel Ballesteros and Valerie Pence. The long-term ex situ conservation of the rich genetic diversity of wild plant species is conventionally achieved by the storage of dry seed at sub-zero temperatures (e.g., -20ºC). However, many species threatened by extinction due to habitat destruct... Read More4 SubsessionsS146 USE OF CRYOBIOTECHNOLOGY FOR THE CONSERVATION OF EXCEPTIONAL AUSTRALIAN MYRTACEAE SPECIES2:00 PM -2:20 PMSpeaker: Lyndle K Hardstaff S147 CRYOPRESERVATION OF SHOOT TIPS FROM QUERCUS BRANDEGEEI, AN EXCEPTIONAL SPECIES FROM MEXICO USING DROPLET-VITRIFICATION2:20 PM -2:40 PMSpeaker: Raquel Folgado S148 HARNESSING CRYOPRESERVATION TECHNIQUES AS A PATH FOR CONSERVING ENDANGERED (ARUM PALAESTINUM): A WILD MEDICINAL PLANT2:40 PM -3:00 PMSpeaker: Rida Shibli S149 (V) THERMAL ANALYSIS AIDS IDENTIFICATION OF MORE APPROPRIATE STORAGE TEMPERATURES FOR RAINFOREST SEEDS SHORT-LIVED AT -20°C3:00 PM -3:20 PMSpeaker: Karen Sommerville

COFFEE BREAK 3:20 PM -4:00 PMat O'Connell Suite

SYMPOSIUM 7: WHOLE ORGANISM CRYOPRESERVATION 4:00 PM -5:40 PMat The Ballroom Co-Chairs: Rebecca Sandlin (V) and Kanav Khosla. Biobanking of whole organisms will have transformative impacts on research and conservation efforts. For researchers, cryopreservation represents an opportunity to bank a growing number of transgenic lines which are susceptible to genetic drift, cross... Read More6 SubsessionsINVITED SPEAKER: S150 (V) RAPID VITRIFICATION STRATEGIES FOR PRESERVATION OF PROTOZOA4:00 PM -4:20 PMSpeaker: Justyna Jaskiewicz INVITED SPEAKER: S151 (V) COMPLEXITIES IN CRYOPRESERVING LATE STAGE EMBRYOS OF THREE DIFFERENT ORDERS OF INSECTS4:20 PM -4:40 PMSpeaker: Arun Rajamohan INVITED SPEAKER: S152 (V) RAPID JOULE HEATING IMPROVES VITRIFICATION BASED CRYOPRESERVATION4:40 PM -5:00 PMSpeaker: Li Zhan INVITED SPEAKER: S153 (V) ROBOTIC PLATFORM FOR AUTOMATED MICROINJECTION OF ZEBRAFISH EMBRYOS FOR CRYOPRESERVATION APPLICATIO5:00 PM -5:20 PMSpeaker: Amey Josi INVITED SPEAKER: S154 (V) 3D PRINTED ORGANISMS FOR HIGH-THROUGHPUT CRYOPRESERVATION5:20 PM -5:40 PMSpeaker: Guebum Han S155 has been moved to Session 6: Cryoprotectants and Ice Formation 25:40 PM -5:40 PM

SESSION 19: RECENT ADVANCES IN CRYOSURGERY 4:00 PM -5:00 PMat Trinity 1 Co-Chairs: John M. Baust (V) and TBA. As medicine moves more towards treatment personalization, there is a pressing need for the development of new strategies, techniques and devices to enable cryoablation to keep pace with the ever-evolving clinical landscape. Further, as evidence-based medicine is... Read More3 SubsessionsS156 (V) THE ROLE OF LAPAROSCOPIC APPROACH IN CRYOABLATION THERAPY FOR INTRA ABDOMINAL MALIGNANCY4:00 PM -4:20 PMSpeaker: Barlian Sutedja S157 (V) COMPARATIVE ANALYSIS OF CRYOABLATION WITH SINGLEHOLE AND MULTIHOLE NOZZLE IN CRYOSPRAY4:20 PM -4:40 PMSpeaker: Prashant Srivastava S158 (V) PREVENTION OF THE INSTABILITY OF THE DORMANT STATE OF TUMOR CELLS IN CANCER STANDARD DIAGNOSTICS4:40 PM -5:00 PMSpeaker: Nikolai Korpan

CLOSING REMARKS 6:00 PM -6:30 PMat The Ballroom Co-Chairs: Greg Fahy and Glyn Stacey. Closing remarks, announcement and presentation of competition winners, and presentation of student travel grant certificates.

CRYO2022 BANQUET 7:00 PM -10:00 PM You won't want to miss this toe tapping Irish music and dance extravaganza.


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